Details
Vit Kit – Freeze NX is an adaptable, cost-effective system for use in the vitrification of oocytes, pronuclear zygotes, cleavage stage embryos, and blastocyst stage embryos. Vit Kit – Freeze NX is the latest advancement in vitrification media aimed to safeguard embryos and achieve high survival rates. Unlike many vitrification kits that feature a mono-buffered system and a M199 base medium originally formulated for chick embryo fibroblasts, Vit Kit – Freeze NX is derived from current IVF media tailored specifically for human embryos.

The backbone of Vit Kit – Freeze NX is Continuous Single Culture medium, an IVF medium intended for conventional fertilization and non-sequential embryo culture. Similar to our Multipurpose Handling Medium, Vit Kit-NX takes advantage of two buffers, HEPES and MOPS, to provide a more secure pH environment for oocytes and embryos during vitrification. Vit Kit – Freeze NX delivers consistent results, including high oocyte and embryo survival rates, while also offering an effective, flexible, and economical media suited for the nuanced needs of the modern IVF laboratory.

Formula based on Continuous Single Culture medium (CSCM) and Multipurpose Handling Medium (MHM) to minimize stress on oocytes and embryos, and achieve high survival rates
Dual-buffered (HEPES and MOPS) to provide a more secure pH environment
Trehalose as the non-permeating cryoprotectant supports higher glass transition temperature and membrane stabilization than sucrose
Dimethyl Sulfoxide (DMSO) and Ethylene Glycol (EG) as permeating cryoprotectants
Supplemented with 20% (v/v) Dextran Serum Supplement (DSS) for a final concentration of 10 mg/mL HSA and 4 mg/mL Dextran
Ready to use for oocyte and embryo vitrification
Contains no phenol red
Compatible with any vitrification device
Catalog No. 90188 Includes:

Equilibration NX – ES (white cap)
3 x 1 mL Vials
Vitrification NX – VS (blue cap)
3 x 1 mL Vials
Washing NX – WS (red cap)
1 x 1 mL Vials

Each kit can be used for up to 50 freezing applications for oocytes and 60 applications for embryos.

Specifications
Shelf Life
12 months from date of manufacture