Details
As one of the most cited density gradient sperm separation media, ISolate is the media of choice for laboratories worldwide to produce clean, robust samples with optimal recovery rates. It is supplied ready-to-use as a dual density gradient solution to process the wide variety of semen samples encountered in the laboratory. The kit, which consists of a 50% upper layer and 90% lower layer, support use with a two layer protocol for healthy samples or with a single layer protocol that enables separation of motile sperm from low quality semen samples. ISolate effectively removes cellular contaminants (dead sperm, white cells, and miscellaneous debris) to yield a sample that contains predominantly progressive, motile sperm, capable of fertilizing the oocyte for IUI, IVF and ICSI.

Formulated with sterile, colloidal suspension of uniform silica particles stabilized with covalently-bound hydrophilic silane in HEPES-buffered HTF
Highest quality raw materials
Quality tested by human sperm survival assay
90% stock and 100% concentrate available for custom dilutions
CE Marked
Quality Control Testing:

Rabbit pyrogen test
Sterility by the current USP, CFR or Ph. Eur. Sterility Test
Sperm Survival Assay
pH
Osmolality

Specifications
Storage
Store at 2-8°C
Shelf Life
2 years from date of manufacture